为了提高用于培养脊髓细胞的产率以及活性,使实验的细胞模型有良好的一致性和可比性.实验探讨了脊髓细胞培养过程中,从取材、解剖到分散细胞等一系列步骤中维持细胞活性的内外因素.应用细胞形态学指标、Trypan blue染色计数分析以及Nissl染色观察结果发现,该改良的方法能够显著提高细胞产率,每条脊髓得到细胞约为3.5～6×10~6个,而且种植不同时间0、4、18、48小时细胞存活率均在95%以上. In order to improve the yield and activity of cultured spinal cord cells, the experimental cell model was well consistent and comparability.The experiment discussed the process of cell culture in spinal cord, from the drawing, dissection to dispersed cells and a series of steps to maintain the cells Activity and internal and external factors.Using cell morphology, Trypan blue staining analysis and Nissl staining results showed that the improved method can significantly improve cell yield, each spinal cord cells obtained about 3.5 ~ 6 × 10 ~ 6, Moreover, the cell survival rates at 0, 4, 18, 48 hours after planting were above 95%.