论文部分内容阅读
目的探讨移植肾组织及血清中透明质酸(HA)、层粘连蛋白(LN)及Ⅳ型胶原(C_(-Ⅳ))含量与急性排斥反应的关系。方法以雄性SD大鼠为供者,Wistar大鼠为受者,采用改进的Blom法制作大鼠原位左肾移植模型,设同种移植对照组、同种移植用药组(术后腹腔注射环孢素A 10 mg·kg~(-1)·d~(-1)),另设同系移植组(供、受者均为Wistar大鼠)和假手术组(Wistar大鼠仅切除右肾,不行移植),每组20只大鼠。每组分别于术后3、5、7及9 d各随机选取5只大鼠,从下腔静脉采血2 ml,随后切取移植肾(假手术组切取左侧肾脏),用放射免疫分析法测定肾组织匀浆及血清中HA、LN及C_(-Ⅳ)的含量,并行肾组织切片,根据“Banff 97”标准进行病理诊断。结果从术后5d开始,同种移植对照组的肾组织匀浆及血清中HA、LN及C_(-Ⅳ)的浓度均显著高于同系移植组、假手术组及同种移植用药组(P＜0．01)；血清中HA、LN及C_(-Ⅳ)的浓度与肾组织匀浆中HA、LN及C_(-Ⅳ)的浓度呈正相关(r分别为0．960、0．934和0．847,P值均为0．000)。病理检查证实,同种移植对照组共有17个肾脏发生急性排斥反应,同种移植用药组共有4个肾脏发生急性排斥反应,它们的Banff急性排斥反应指数与移植肾组织匀浆中及血清中HA、LN及C_(-Ⅳ)的浓度均呈正相关(P＜0．01)。结论HA、LN及C_(-Ⅳ)在肾移植急性排斥反应的发生及发展中起重要作用,肾移植术后监测血清中HA、LN及C_(-Ⅳ)的浓度有助于急性排斥反应的诊断。
Objective To investigate the relationship between the levels of hyaluronic acid (HA), laminin (LN) and type Ⅳ collagen (C_ (Ⅳ)) in renal allograft and serum and acute rejection. Methods Male Sprague-Dawley rats were used as donors and Wistar rats as recipients. The rats were treated with improved Blom method to establish orthotopic left renal allograft model. Allograft control group, allograft group (Wistar rats) and sham operation group (Wistar rats, only right kidney, Not transplant), each group of 20 rats. Five rats were randomly selected from each group at 3, 5, 7, and 9 days after operation. Blood samples were drawn from the inferior vena cava and then transplanted to the kidney (sham operation group, left kidney). Radioimmunoassay The content of HA, LN and C_ (Ⅳ) in the homogenate and serum of the rats were analyzed by histological sections. The pathological diagnosis was performed according to the “Banff 97” standard. Results From the 5th day after operation, the concentrations of HA, LN and C_ (- Ⅳ) in the kidney homogenate and serum in the allograft control group were significantly higher than those in the homograft group, the sham operation group and the allograft group (P <0.01). The concentrations of HA, LN and C_ (- Ⅳ) in serum were positively correlated with the levels of HA, LN and C_ (- Ⅳ) in renal homogenate (r = 0.960,0.934 and 0.847, P values were 0.000). Pathological examination confirmed that allograft control group, a total of 17 renal acute rejection, allograft group, a total of four renal acute rejection, their Banff acute rejection index and transplantation of renal homogenate and serum HA , LN and C (- Ⅳ) concentrations were positively correlated (P <0.01). Conclusions HA, LN and C_ (- Ⅳ) play an important role in the occurrence and development of acute rejection in renal allografts. Monitoring serum concentrations of HA, LN and C_ (- Ⅳ) after renal transplantation may contribute to acute rejection diagnosis.