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目的:探讨通天草水提物(AEWH)对APP/PS1双转基因小鼠海马的保护作用。方法:雄性小鼠随机分成对照组,模型组,多奈哌齐组(多奈哌齐0.00092 g·kg~(-1)),AEWH高、中、低剂量组(AEWH 2.5、1.25、0.63 g·kg~(-1))。灌胃给予相同体积的药物或生理盐水,每天灌胃1次,持续35 d。采用Morris水迷宫实验观察小鼠的学习记忆能力,检测小鼠脏器指数,采用双抗体夹心法测定海马白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量。结果:与模型组比较,AEWH能明显改善APP/PS1双转基因小鼠的学习记忆能力(P<0.05),明显升高脾和胸腺脏器指数(P<0.05),明显降低海马IL-1β和TNF-α含量(P<0.05)。结论:AEWH能改善APP/PS1双转基因小鼠的学习记忆能力及脾和胸腺脏器指数,机制与降低海马IL-1β和TNF-α水平有关。
Objective: To investigate the protective effect of the extract of ChuanTian Cao (AEWH) on the hippocampus of APP / PS1 double transgenic mice. Methods: Male mice were randomly divided into control group, model group, donepezil group (doneonexetil 0.00092 g · kg -1), AEWH high, middle and low dose groups (AEWH 2.5,1.25,0.63 g · kg -1 )). Gavage given the same volume of drugs or saline, gavage once daily for 35 d. The Morris water maze test was used to observe the learning and memory abilities of mice. The organ index of mice was measured. The content of IL-1β and TNF-α in the hippocampus was determined by double antibody sandwich method. Results: Compared with the model group, AEWH significantly improved the learning and memory abilities of APP / PS1 double transgenic mice (P <0.05), significantly increased the index of spleen and thymus organs (P <0.05) and significantly decreased the levels of IL-1β TNF-α content (P <0.05). CONCLUSION: AEWH can improve the learning and memory ability and the index of spleen and thymus organ in APP / PS1 double transgenic mice. The mechanism is related to the decrease of IL-1β and TNF-α in hippocampus.