论文部分内容阅读
本文应用PCR技术从人基因组DNA中扩增出胰岛素基因片段(1128bp),将扩增产物直接克隆到M_(13)载体中去,用双脱氧链终止法作DNA序列分析。是鉴定胰岛素基因中前肽、A链、B链和C肽编码区域有无碱基突变的一种简便、有效的方法
In this paper, the insulin gene fragment (1128bp) was amplified from human genomic DNA by PCR. The amplified product was directly cloned into the M13 vector and analyzed by dideoxy chain termination method. Is a simple and effective method to identify the presence or absence of a base mutation in the propeptide, A chain, B chain and C peptide in the insulin gene