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采用紫外分光光度法测定土霉素片的含量。以盐酸液（0．1mol／L）为溶剂，检测波长为353nm，土霉素的浓度10～26μg／ml，与吸收度呈线性相关（r=0．9999；回归方程C=32．452A＋0．0037），回收率为100．1％，相对标准偏差为0．15％（n=6）。与抗生素微生物检定法做比较试验，两种分析方法间无显著性差异。本法简便、准确，适用于常规分析。
Determination of oxytetracycline tablets by UV spectrophotometry. With hydrochloric acid (0.1 mol / L) as solvent, the detection wavelength was 353 nm and the concentration of oxytetracycline was 10-26 μg / ml, which was linearly correlated with the absorbance (r = 0.9999; regression equation C = 32.452A + 0037). The recovery was 100.1% and the relative standard deviation was 0.15% (n = 6). Compared with the antibiotic microbial test, there was no significant difference between the two methods. This method is simple, accurate and suitable for routine analysis.