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本研究通过hMeDIP-Seq技术和生物信息学分析,从肝细胞癌模型小鼠肝癌组织和肝组织基因组羟甲基化的角度研究肝癌相关信号通路,以期探讨肝癌相关信号分子基因的羟甲基化分布及其与基因表达的关系。研究结果表明,肝癌组织中共有52个具有显著统计学意义的肝癌相关信号分子基因发生了羟(FDR<0.05),而肝组织的则没有出现有统计学意义的相关羟甲基化信号分子基因。而且,这些信号分子基因的羟甲基化位点全部分布在基因表达的调控区域(外显子外),包括启动子-转录启始位点区、内含子区和基因间区。由于DNA羟甲基化是激活基因表达的重要手段,因此,这些区域的羟甲基化表明这些基因在肝癌组织中处于激活状态,并得到了RT-q PCR实验的抽样证明。由此可见,肝癌组织基因组上发生的羟甲基化与肝癌相关;它们通过激活大量的信号分子基因引起肝癌相关信号通路的异常活化,从而引起肝癌的发生或迁移。
In this study, by using hMeDIP-Seq technology and bioinformatics analysis, the hepatoma-related signaling pathways were studied from the perspective of methylation of hepatocellular carcinoma tissue and liver tissue in hepatocellular carcinoma mouse model to investigate the methylation of hepatocellular carcinoma related signal molecules Distribution and Its Relationship with Gene Expression. The results showed that a total of 52 hepatocellular carcinoma-related signal molecule genes were found to have hydroxyl (FDR <0.05) in liver cancer tissues, while no statistically significant correlation was found with liver methylation . Moreover, the methylation sites of these signaling molecules are all located in the regulatory region of gene expression (exon), including the promoter-transcription initiation site region, intron region and intergenic region. As DNA methylation is an important means of activating gene expression, methylation of these regions suggests that these genes are activated in HCC and have been sampled from RT-q PCR experiments. Thus, the occurrence of methylation of HCC tissue associated with liver cancer; they cause abnormal activation of liver cancer-related signaling pathways by activating a large number of signal molecule genes, resulting in the occurrence or migration of liver cancer.