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为获得对香蕉枯萎病菌具有稳定拮抗作用的微生物菌株,分别从海南省临高县南宝镇新营农场、皇桐村及美台镇美梅村采集感病土壤和健康土壤进行微生物分离,并以香蕉枯萎病菌4号小种(Fusarium oxysporum f.sp.cubense Race 4)等9种病原菌为靶标菌株,采用平板对峙法和含药介质法对拮抗菌的抑菌活性进行评价,同时根据形态特征、培养特征及生理生化特征和16S rDNA序列分析对其进行鉴定。结果表明:6个土壤样品中共分离得到具有拮抗作用的细菌93株和放线菌133株,其中T3-G-59菌株对9种病原菌具有广谱抗性,菌丝生长抑制率为43.58%~86.43%,孢子萌发抑制率为42.22%~81.11%,其中对香蕉枯萎病菌4号小种的菌丝生长和孢子萌发抑制率分别为86.43%和81.00%,经鉴定该菌株为多产色链霉菌Streptomyces polychromogenes,表明该多产色链霉菌T3-G-59菌株具有应用于香蕉枯萎病生物防治的潜力。
In order to obtain the strain of microorganism with stable antagonism against Fusarium oxysporum f. Sp., Microorganisms were isolated from the susceptible and healthy soils of Xinying Farm, Huangtong Village, Nanba Town, Lincoli County, Hainan Province and MeiMei Village, Meitai Town, respectively, Nine pathogenic bacteria, Fusarium oxysporum fspspubense Race 4, were selected as the target strains. The antibacterial activity of the antagonistic bacteria was evaluated by plate confrontation method and drug-mediated method. Meanwhile, according to the morphological characteristics, Culture characteristics and physiological and biochemical characteristics and 16S rDNA sequence analysis of their identification. The results showed that there were 93 bacterial strains and 133 actinomycetes isolated from 6 soil samples. Among them, T3-G-59 strains had broad-spectrum resistance to 9 pathogenic bacteria, and mycelial growth inhibition rate was 43.58% 86.43%, the inhibition rate of spore germination was 42.22% ~ 81.11%. The inhibitory rates of mycelial growth and spore germination of F4 were 86.43% and 81.00%, respectively. The strain was identified as Streptomyces polyornis Streptomyces polychromogenes, indicating that the strain of Streptomyces polytophilum T3-G-59 has the potential for biological control of banana wilt disease.