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HIV- 1Rev是一个 RNA核输因子。它通过 Rev ARM与 RNA相结合 ,并且通过 Rev NES与连有Ran GTP的 Crml分子结合 ,然后通过 Crml与特异核孔蛋白的依次作用 ,进而导致未拼接或未完全拼接的病毒RNA转运到核外。在许多病毒和细胞的蛋白中都已鉴定出含有 NES,这些 NES具有共同的序列特征 :L—— X( 2 -3)—— L( I、M、V、F)—— X( 2 - 3)—— L—— X—— L( I) ,但也不能仅凭借序列的同源性就确定 NES功能。要鉴定一段序列在蛋白中是否具有 NES功能 ,主要还要看它是否能介导它所在蛋白的核输出。简单的逆转录病毒不能编码有 Rev同等作用的核 RNA输出因子 ,但它能把细胞的核输出信号 ( CTE)融入到自己的基因组 RNA中 ,从而指导未拼接或未完全拼接的病毒 RNA转运到核外。CTE是目前最易理解的一个病毒 RNA要素 ,它不但可以参与对病毒 m RNA而且还能够直接参与对细胞 m RNA核输出的调控
HIV-1Rev is an RNA negative factor. It binds to RNA via Rev ARM and binds to Ran GTP-linked Crml through Rev NES and then transfers unlabeled or incompletely spliced viral RNA to the nucleus through the sequential interaction of Crml and specific nuclear porins . Containing NES has been identified in many viruses and cell proteins and these NESs share common sequence features: L - (2 - 3) - L (I, M, V, F) 3) - L - X - L (I), but NES functionality can not be determined by virtue of the sequence homology alone. To identify whether a sequence has a NES function in a protein depends mainly on whether it can mediate the nuclear export of its protein. A simple retrovirus can not encode a nuclear RNA exporter with the same role of Rev, but it can incorporate the nuclear export signal (CTE) of a cell into its own genomic RNA to direct the unlinked or incompletely spliced viral RNA to Nuclear. CTE is currently one of the most understandable viral RNA elements that not only can be involved in viral m RNA but also can be directly involved in the regulation of cellular m RNA nuclear export