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目的探讨白细胞介素-4(IL-4)基因多态性与儿童哮喘患病风险的关系。方法采用以人群基础的病例对照研究(293例哮喘儿童和668例对照儿童)方法 ,进行IL-4C-590T基因多态与儿童哮喘风险关联分析,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)进行基因分型。结果未发现IL-4C-590T多态性与儿童哮喘显著关联,但按照性别分层后,在女童中CC基因型携带者哮喘患病危险性显著降低,OR值为0.10(95%CI:0.01～0.77)。IL-4-590CC和CT基因型与饲养鸽子或其他鸟类两因素共同存在时,有明显的正向交互作用,产生交互作用的ORint值为2.562(P=0.008),哮喘患病风险中可以归因于两因素交互作用的比例(AP)占63%,交互作用指数(S)达到1.392。IL-4-590CC和CT基因型与被动吸烟也存在正向交互作用,ORint值为1.836(P=0.064),达到临界显著性。结论在女童中发现IL-4C-590T多态性与儿童哮喘患病风险显著关联,并且该基因变异与饲养鸽子或其他鸟类、被动吸烟存在正向交互作用。
Objective To investigate the relationship between interleukin-4 (IL-4) gene polymorphism and childhood asthma risk. Methods A population-based case-control study (293 asthmatic children and 668 control children) was performed to analyze the association between IL-4C-590T polymorphism and childhood asthma. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for genotyping. Results No significant association was found between IL-4C-590T polymorphism and childhood asthma. However, stratification according to gender had a significantly lower prevalence of asthma in girls with CC genotype (OR = 0.10, 95% CI: 0.01 ~ 0.77). There was a significant positive interaction between IL-4-590CC and CT genotypes and pigeons or other birds, with an OR of 2.562 (P = 0.008). The risk of asthma was The proportion (AP) attributable to the two-factor interaction accounted for 63% and the interaction index (S) reached 1.392. There was also a positive interaction between IL-4-590CC and CT genotypes and passive smoking with an OR of 1.836 (P = 0.064), reaching a critical significance. Conclusion The IL-4C-590T polymorphism was found to be significantly associated with the risk of childhood asthma in girls and there was a positive interaction between this gene variation and rearing of pigeons or other birds and passive smoking.