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为观察二甲基砷(DMAV)染毒后的大鼠膀胱上皮细胞增殖相关因子表达情况,将48只初断乳健康Wistar大鼠随机分成对照组和低(100 ppm)、高(200 ppm)剂量DMAV染毒组,雌雄各半。染毒组饮用含DMAV的水,对照组饮用蒸馏水,染毒10周后,通过免疫组化法观察大鼠膀胱上皮细胞PCNA、cyclinD1、COX-2表达情况。结果显示各染毒组PCNA、cyclinD1蛋白表达均显著升高(P<0.01),200ppm DMAV染毒组COX-2蛋白表达显著升高(P<0.05)。提示DMAV对大鼠膀胱上皮细胞具有促增殖作用。
To observe the expression of proliferation related factors of rat bladder epithelial cells after exposure to dimethyl arsenic (DMAV), 48 healthy weanling Wistar rats were randomly divided into control group and low (100 ppm) and high (200 ppm) Dose DMAV exposure group, male and female. Rats in the exposure group received water containing DMAV, while those in the control group received distilled water. After 10 weeks of exposure, the expression of PCNA, cyclinD1 and COX-2 in bladder epithelial cells of rats were observed by immunohistochemistry. The results showed that the expression of PCNA and cyclinD1 in each group were significantly increased (P <0.01), and the expression of COX-2 protein in 200ppm DMAV group was significantly increased (P <0.05). Tip DMAV on rat bladder epithelial cells have a role in promoting proliferation.