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为了探索绵羊产羔性状与绵羊BMPR-IB基因多态性位点关系,找寻调控绵羊繁殖力的分子标记,以小尾寒羊(多胎母羊)、湖羊(多胎母羊)、蒙古羊(单,双胎母羊)、和甘肃高山细毛羊(单,双胎母羊)样本为试验材料,运用DNA直接测序及PCR-SSCP技术的方法,对BMPR-IB基因CDS区864位点进行分析。试验羊品种出现3种基因型AA、AB、BB,该基因编码区第846位点发生T>C的突变。湖羊母羊以及小尾寒羊母羊的优势基因型均为AA型,而蒙古羊母羊和甘肃高山细毛羊母羊AB型基因频率略高于AA型。4种绵羊的优势等位基因均为A。小尾寒羊、甘肃高山细毛羊、湖羊三种绵羊x2值和G2值均未达到显著水平(p>0.05),而蒙古羊的x2值和G2值达到显著水平(p<0.05),说明除了蒙古羊其他3种羊均达到Hardy-weinberg平衡状态。四种绵羊的观测值F在BMPR-IB基因中均处于95%置信区间内,且接近于上线。根据各品种间的PIC多态信息含量可知,属于低度多态的是小尾寒羊和湖羊(PIC<0.25),而在蒙古羊和甘肃高山细毛羊信息含量处于中度多态(0.25 C occurred at the 846th of the coding region. The dominant genotypes of Hu sheep and Small Tail Han sheep were all AA genotype, while the frequencies of AB genotypes in Mongolia sheep and Gansu alpine sheep were slightly higher than those in AA genotype. The dominant alleles of the four species of sheep are A. Small sheep, Gansu alpine fine wool sheep, Hu sheep three sheep x2 value and G2 value did not reach a significant level (p> 0.05), while Mongolia sheep x2 value and G2 value reached significant level (p <0.05), indicating that in addition to Mongolian sheep and other three kinds of sheep have reached Hardy-weinberg equilibrium. Observations F for the four sheeps were all within the 95% confidence interval for the BMPR-IB gene and were close to going online. According to PIC polymorphism information content of all breeds, it is known that small polymorphism is Small Tailed Han sheep and Hu sheep (PIC <0.25), while Mongolian sheep and Gansu Alpine fine wool sheep are moderately polymorphic (0.25 < PIC <0.5). Significant test analysis showed that there was a significant difference (p <0.05) between Gansu alpine fine wool sheep and Small Tail Han sheep, Hu sheep, Mongolian sheep, Small Tail Han sheep and Hu sheep. The mutation at position 846 in the coding region of BMPR-IB gene was distributed in different breeder ewes and could be used as a potential molecular marker for sheep.