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以肉质叶为外植体,研究文采唇柱苣苔的组织培养和快速繁殖。结果表明:培养基MS+6-BA 0.1 mg?L-1+NAA 0.1mg?L-1适用于初代培养时的愈伤组织诱导和植株再生;MS+6-BA 0.5 mg?L-1+NAA 0.2 mg?L-1+10%香蕉泥和MS+6-BA 0.5mg?L-1+NAA 0.5 mg?L-1+10%香蕉泥分别适用于继代增殖及壮苗培养,60 d后的增殖系数分别为6.7和4.8;培养基MS适用于生根培养,培养30 d,生根率达100%。另外,对文采唇柱苣苔生根试管苗进行大棚移栽,移栽成活率约为94%。
The fleshy leaves were used as explants to study the tissue culture and rapid propagation of Pleurotus ostreatus. The results showed that MS + 6-BA 0.1 mg? L-1 + NAA 0.1 mg? L-1 was suitable for callus induction and plant regeneration in primary culture. MS + 6-BA 0.5 mg? L-1 + NAA 0.2 mg? L-1 + 10% banana puree and MS + 6-BA 0.5 mg? L-1 + NAA 0.5 mg? L-1 + 10% banana puree were suitable for subculture and seedling culture respectively, After multiplication, the multiplication coefficients were 6.7 and 4.8, respectively. The medium of MS was suitable for rooting and culture for 30 days with rooting rate of 100%. In addition, the literal lip column bolets in vitro test tube plantlets transplanting, transplanting survival rate of about 94%.