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目的研究苏丹红Ⅰ对小鼠肺组织的损伤及肺组织p53和Ki-67表达的影响。方法昆明小鼠40只,随机分为4组:空白对照组,苏丹红Ⅰ低剂量(60 mg/kg)和苏丹红Ⅰ中剂量(120 mg/kg),苏丹红Ⅰ高剂量(240 mg/kg)组,10只/组。连续胃灌两周,于两周末处死,HE染色观察病理变化,免疫组化法检测肺组织p53和Ki-67表达。结果与对照组比较,实验组小鼠的肺泡隔增厚,且随着苏丹红Ⅰ浓度的增加,可见部分支气管充血和炎症细胞浸润;Ki-67在各组中表达差异有统计学意义(F=68.26,P<0.001),中浓度、高浓度组与对照组比较表达降低(均有P<0.05),p53在各组中表达差异有统计学意义(F=23.26,P<0.001),各浓度组与对照组比较表达增加(均有P<0.05)。结论苏丹红Ⅰ通过增加凋亡,抑制增殖来损伤小鼠肺组织。
Objective To study the effects of Sudan Ⅰ on the lung injury and the expression of p53 and Ki-67 in lung tissue of mice. Methods Forty Kunming mice were randomly divided into four groups: blank control group, low dose of Sudan Ⅰ (60 mg / kg) and middle dose of Sudan Ⅰ (120 mg / kg), high dose of Sudan Ⅰ (240 mg / kg) group, 10 / group. The rats were sacrificed after two weeks of continuous gastric perfusion. The pathological changes were observed by HE staining and the expressions of p53 and Ki-67 were detected by immunohistochemistry. Results Compared with the control group, the alveolar septa of mice in experimental group were thickened, and some bronchial congestion and infiltration of inflammatory cells were observed with the increase of Sudan Ⅰ concentration. The expression of Ki-67 in each group was statistically significant (F = 68.26, P <0.001). The expression of p53 in each group was significantly lower than that in the control group (both P <0.05) Concentration group compared with the control group increased (P <0.05). Conclusion Sudan Ⅰ can injure mice lung by increasing apoptosis and inhibiting proliferation.