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本文报导了测定香蕉中甲基托布津及其活性代谢物残留量的薄层—生物测定方法。该法与其它分析方法比较,具有不需要复杂的净化步骤,在薄层板上可根据同时显现的原药及活性代谢物的抑菌圈进行定性、定量测定;操作简便,并且不需要贵重仪器设备。尤其适用于植物体内外进行活化代谢的杀菌剂残留量测定。该法在香蕉中的最低检出界限为0.1ppm,回收率95%以上,符合常规测定要求。实测结果证明,来自广西、广东的香蕉样本,总残留量不超过 FAO/WHO 推荐的最大残留允许标准1ppm。
This paper reports a thin-layer bioassay for the determination of methyl thiophanate and its active metabolite residues in bananas. Compared with other analytical methods, this method does not require complicated purification steps, and can be qualitatively and quantitatively determined on the thin-layer plate according to the antibacterial circle of the original drug and the active metabolite simultaneously. The method is easy to operate and does not require expensive instruments equipment. It is especially suitable for the determination of bactericide residues in plants for activation and metabolism in vitro and in vivo. The method in bananas in the minimum detection limit of 0.1ppm, the recovery rate of 95% or more, in line with routine testing requirements. The measured results show that the banana samples from Guangxi and Guangdong, the total residue does not exceed the maximum residue allowable standard FAO / WHO 1ppm.