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本实验用~3H—TdR脉冲标记放射自显影技术,对第19天小白鼠S_(180)腹水型细胞进行了实验分析,并观察了亚显微结构。发现各型S_(180)细胞均具有DNA复制功能,胞质内常见含有成群存在的“病毒样小体”;并发现光镜下所见的所谓“畸形核”,实际上大都是多叶核。认为哑铃形核细胞及多叶核细胞,大部分属于无丝分裂期细胞。本实验结果与用低渗处理法的观察结果一致。
In this study, ~ 3H-TdR pulsed-labeled autoradiography was used to analyze the S_ (180) ascites cells on day 19 in mice and to observe the sub-microstructure. Found that all types of S 180 cells have DNA replication function, the cytoplasm often contains clusters of “virus-like bodies”; and found that the light microscope, the so-called “abnormal nuclei”, in fact, mostly multi-leaf nuclear. Dumbbell nucleus cells and multi-lobe cells, most of which belong to amitotic cells. The experimental results with the hypotonic treatment of the observations consistent.