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雌性大鼠随机分为 4组 ,腹腔注射氯化锰两周 ,隔日一次 ,剂量分为 0、7 5、15、3 0mg/kg。受孕后以同样方法继续染锰一周。分娩后对其新生鼠肝组织用western斑点印迹法检测热应激蛋白 70 (HSP70 ) ,并测定其超氧化物歧化酶(SOD)。发现 15mg/kg组SOD活性低于对照组 (P <0 0 5 ) ;HSP70含量 ,染锰各组明显高于对照组 (P <0 0 1)。提示亲代妊娠期染锰可诱导子代新生鼠肝组织HSP70合成增加 ,但这种效应在锰的胚胎发育毒性中的作用和机理仍有待进一步阐明。
Female rats were randomly divided into 4 groups, intraperitoneal injection of manganese chloride two weeks, every other day, the dose was divided into 0,7 5,15,3 0 mg / kg. Continue to dye the manganese for one week in the same way after conception. After delivery, heat shock protein 70 (HSP70) was detected by Western blotting in liver tissue of newborn rats and the superoxide dismutase (SOD) was measured. The SOD activity in 15mg / kg group was lower than that in control group (P <0.05). The content of HSP70 in each group was significantly higher than that in control group (P <0.01). It is suggested that the increase of HSP70 synthesis in neonatal rat liver tissue induced by manganese during pregnancy may be induced by maternal pregnancy. However, the role and mechanism of this effect in embryonic developmental toxicity of manganese remains to be elucidated.