目的建立降脂宁有效部位中4种蒽醌类成分橙黄决明素、大黄素、大黄酚、大黄素甲醚的HPLC含量测定方法。方法采用SunFire C_(18)柱(150 mm×4.6 mm,5μm),柱温30℃,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速为1.0 mL/min,检测波长285 nm。结果橙黄决明素、大黄素、大黄酚、大黄素甲醚平均回收率分别为98.69%、99.97%、97.98%和99.20%,RSD分别为2.20%、1.18%、2.27%和1.15%。结论3批样品测定结果表明,该方法简便、准确,可用于降脂宁有效部位中4种蒽醌类成分的含量测定。 OBJECTIVE To establish a HPLC method for the determination of 4 anthraquinone components, namely, Huang Huang Jiming Ming, emodin, chrysophanol and physcion in the effective part of Jiangzhining. The method was performed on a SunFire C_ (18) column (150 mm × 4.6 mm, 5 μm) with a column temperature of 30 ° C. The mobile phase consisted of acetonitrile-0.1% phosphoric acid in water with a gradient of 1.0 mL / min and a detection wavelength of 285 nm. Results The average recoveries of emodin, emodin, chrysophanol and physcion were 98.69%, 99.97%, 97.98% and 99.20%, respectively. The RSDs were 2.20%, 1.18%, 2.27% and 1.15%, respectively. Conclusion The results of three batches of samples show that the method is simple and accurate and can be used to determine the content of four anthraquinones in the effective part of Jiangzhining.