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目的探讨大鼠牙周炎与高脂血症的关系。方法本实验于2005年6月至2011年6月在福建省高校口腔医学重点实验室及中国人民解放军南京军区福州总医院比较医学科完成。40只雄性SD大鼠按体重随机分为A、B、C、D组,每组10只。分别进行如下处理:A组,正常饲养;B组,单纯牙周结扎;C组,单纯高脂饮食;D组,牙周结扎+高脂饮食。3个月后处死大鼠,检测血清胆固醇(CHO)、甘油三酯(TG)、高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)浓度;同时,取大鼠下颌第一磨牙做组织学观察分析。结果 (1)牙周结扎大鼠磨牙牙槽骨出现不同程度的骨吸收。(2)各组大鼠血清TG浓度差异无统计学意义(P>0.05);高脂饮食大鼠血清CHO、HDL-C和LDL-C浓度均明显高于正常饮食大鼠,且D组大鼠血清CHO浓度高于C组(均P<0.05);C、D组HDL-C和LDL-C浓度差异无统计学意义(P>0.05)。结论通过高脂喂养和磨牙牙周结扎可建立高脂血症大鼠实验性牙周炎动物模型,牙周炎可致高脂饮食大鼠血清CHO浓度升高。
Objective To investigate the relationship between periodontitis and hyperlipidemia in rats. Methods The experiment was performed from June 2005 to June 2011 in Key Laboratory of Stomatology of Fujian Province and Department of Comparative Medicine of Fuzhou General Hospital of Nanjing Military Region, People’s Liberation Army of China. Forty male SD rats were randomly divided into groups A, B, C and D according to body weight, with 10 rats in each group. The following treatments were performed: Group A, normal feeding; Group B, simple periodontal ligature; Group C, simple high fat diet; Group D, periodontal ligation + high fat diet. After 3 months, the rats were sacrificed and the concentrations of serum cholesterol (CHO), triglyceride (TG), high density lipoprotein (HDL-C) and low density lipoprotein (LDL- A molars histological observation and analysis. Results (1) Periodontal ligation rat molar alveolar bone varying degrees of bone resorption. (2) There was no significant difference in the concentration of serum TG in each group (P> 0.05). The concentrations of serum CHO, HDL-C and LDL-C in high fat diet group were significantly higher than those in normal diet group The concentration of serum CHO was higher in group C than in group C (all P <0.05). There was no significant difference in HDL-C and LDL-C between group C and group D (P> 0.05). Conclusion The animal model of experimental periodontitis in hyperlipidemic rats can be established by periodontal ligation with high-fat diet and periodontitis, and periodontitis can cause elevated serum CHO concentrations in rats fed high-fat diet.