We experimentally demonstrate the observation of macroscopic resonant tunneling (MRT) phenomenon of the macroscopic distinct flux states in a radio frequency superconducting quantum interference device (rf-SQUID) under a singlecycle sinusoidal driving.The population of the qubit exhibits interference pattes corresponding to resonant tunneling peaks between states in the adjacent potential wells.The dynamics of the qubit depends significantly on the amplitude,frequency,and initial phase of the driving signal.We do the numerical simulations considering the intra-well and interwell relaxation mechanism,which agree well with the experimental results.This approach provides an effective way to manipulate the qubit population by adjusting the parameters of the exteal driving field.