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目的:建立二陈汤中橙皮苷、甘草酸和甘草次酸的高效毛细管电泳(HPCE)含量测定方法。方法:采用高效毛细管电泳法,电泳条件为未涂层石英毛细管柱50μm×95 cm(有效长度86.5 cm),运行缓冲液50 mmol.L-1硼砂溶液(含10%乙腈,pH 9.28);压力进样50 kPa×20 s,电压为24 kV,柱温20℃,检测波长254 nm。结果:橙皮苷、甘草酸与甘草次酸分别在0.10～2.40 g.L-1,0.04～0.72 g.L-1,0.01～0.18 g.L-1呈良好线性关系,r分别为0.999 4,0.999 4,0.999 6,平均回收率分别为98.17%,98.72%,98.48%,RSD分别为1.49%,2.95%,2.25%。结论:该方法简便、快速、准确,在一定程度上弥补了高效液相法的不足,为二陈汤电泳指纹图谱的建立提供了依据,并为该方药效物质基础的研究奠定了基础。
Objective: To establish a method for the determination of hesperidin, glycyrrhizic acid and glycyrrhetinic acid in Erchen Tang by high performance capillary electrophoresis (HPCE). Methods: High-performance capillary electrophoresis was used. The electrophoresis conditions were 50 μm × 95 cm (effective length 86.5 cm) and 50 mmol·L-1 borax buffer (containing 10% acetonitrile, pH 9.28) The injection was 50 kPa × 20 s, the voltage was 24 kV, the column temperature was 20 ℃ and the detection wavelength was 254 nm. Results: There was a good linear relationship between hesperidin, glycyrrhizin and glycyrrhetinic acid in the range of 0.10-2.40 gL-1.0.04-0.72 gL-1 and 0.01-1.18 gL-1, respectively, r = 0.999 4,0.999 4,0.999 6 The average recoveries were 98.17%, 98.72% and 98.48%, respectively, with RSDs of 1.49%, 2.95% and 2.25%, respectively. Conclusion: The method is simple, rapid and accurate, which can make up for the deficiency of high performance liquid chromatography to a certain extent. It provides the basis for the establishment of electrophoresis fingerprinting of Erchen Tang, and lays the foundation for the study of the material basis of this prescription.