论文部分内容阅读
为了满足基础和临床病毒学研究的需要,对人甲型肝炎病毒(HAV)进行了细胞培养。经30多年的努力,至1979年才由Provost和Hilleman首次报告应用狨猴肝和恒河猴胎肾细胞进行HAV培养获得成功。同年Fr(?)sner用人肝癌细胞株培养HAV。但由于该细胞株不易得到,加之HAV在该细胞上生长缓慢,故受到一定限制。本文推荐使用常规细胞株培养HAV,可克服上述缺点。材料①粪便标本取自1978年Kyto流行甲型肝炎时所得的粪便粗提物。②细胞用人羊膜细胞株(FL)和非洲绿猴肾细胞株(Vero)。③营养液是含10%胎牛血清和60μg/ml卡那霉素的Eagle液。④维持液用含2%胎牛血清的Eagle液。方法①将FL和Vero细胞培养成单层后,接
To meet the needs of basic and clinical virology studies, human Hepatitis A virus (HAV) was subjected to cell culture. After more than 30 years of hard work, it was first reported by Provost and Hilleman in 1979 that application of marmoset and rhesus fetal kidney cells for HAV culture was successful. In the same year, Fr (?) Sner cultured human hepatoma cell line HAV. However, due to the difficulty of obtaining the cell line and the slow growth of HAV on the cell, it is limited. This article recommends the use of conventional cell lines cultured HAV, to overcome the above shortcomings. Materials (1) Stool specimens were taken from the excrement extracts obtained from Kyto’s pandemic hepatitis A in 1978. ② human amniotic cell line (FL) and African green monkey kidney cell line (Vero). ③ nutrient solution is 10% fetal calf serum and 60μg / ml kanamycin Eagle fluid. ④ maintenance solution containing 2% fetal bovine serum Eagle fluid. Methods ① FL and Vero cells were cultured into a single layer, then