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常规测定总叶绿素浓度是用丙酮提取离析植物组织,然后通过分光光度计分别测定提取液中叶绿素a和叶绿素b的吸收光谱(Arnon,1949)。然而,丙酮提取液不稳定,而且分析大量样品时离析和分光光度计测定都很费时。最近报导了利用二甲基亚矾(DMSO)从完整植物材料中提取叶绿素的方法(Hiscox等,1979),以及通过液体闪烁计数器进行糖分和
Routine determination of total chlorophyll concentration is extracted by acetone extraction of plant tissue, and then by spectrophotometer were measured in the extract of chlorophyll a and chlorophyll b absorption spectra (Arnon, 1949). However, acetone extract is not stable, and it is time-consuming to analyze and analyze spectrophotometers when analyzing a large number of samples. Recently methods for the extraction of chlorophyll from intact plant material using dimethyl sulfoxide (DMSO) (Hiscox et al., 1979) as well as the use of liquid scintillation counters for sugar and