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目的探讨亚致死剂量球形芽孢杆菌(Bacillus sphaericus,Bs)对斯氏按蚊传疟能力的影响。方法利用亚致死剂量Bs 2 362株处理斯氏按蚊Ⅳ龄幼虫,收集存活按蚊并常规饲养。正常对照组按蚊Ⅳ龄幼虫不进行Bs处理,常规饲养。对3~5 d龄的斯氏按蚊成蚊进行约氏疟原虫攻击感染,并于按蚊吸血后第9天解剖蚊胃,观察卵囊的发育情况,比较分析Bs处理对斯氏按蚊传疟能力的影响。结果正常对照组和Bs处理组按蚊对疟原虫的感染率分别为92.1%和97.6%,Bs处理组感染率高于正常对照组,差异有统计学意义(P<0.05)。Sigma 3.5统计软件分析显示,正常对照组和Bs处理组斯氏按蚊对疟原虫的感染度分别为(56.69±69.29)和(84.19±75.64),Bs处理组按蚊卵囊数量显著高于正常对照组,差异有统计学意义(P<0.05)。结论亚致死剂量Bs 2362株处理显著增加约氏疟原虫对斯氏按蚊的感染率和感染度,提高了按蚊的传疟能力。
Objective To investigate the effects of sublethal dose Bacillus sphaericus (Bs) on malaria parasiticus. Methods The fourth instar larvae of Anopheles stephensi were treated with a sublethal dose of Bs 2 362 and the surviving Anopheles mosquitoes were collected and routinely housed. The normal control group Anopheles Ⅳ instar larvae were not Bs treatment, routine feeding. Infected with P. yoanalis 3 ~ 5 d-old adult mosquitoes, and mosquitoes were dissected on the 9th day after the anopheles sucked blood, the development of oocysts was observed, The impact of malaria transmission capacity. Results The infection rate of Plasmodium in the control group and Bs treatment group was 92.1% and 97.6%, respectively. The infection rate of Bs in treatment group was higher than that in normal control group (P <0.05). Sigma 3.5 statistical software analysis showed that the infection rates of Plasmodium parahaemolyticus in the normal control group and Bs treatment group were (56.69 ± 69.29) and (84.19 ± 75.64), respectively, and those in the Bs treatment group were significantly higher than those in the normal Control group, the difference was statistically significant (P <0.05). Conclusion Sublethal dose of Bs 2362 strain significantly increased the infection rate and infection rate of Anopheles stephensi to Anopheles stephensi and increased the malaria transmission ability of Anopheles.