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本研究的目的是观察预激态补骨脂素对K5 6 2细胞增殖的影响 ,为补骨脂素的临床应用提供实验依据。取预激态补骨脂素和晚激态补骨脂素处理的细胞 ,在培养后检测台盼蓝拒染细胞数和白血病细胞集落 ,并对它们在台盼蓝拒染细胞抑制率 (TBIR)、细胞增殖抑制率 (CPIR)和集落形成抑制率 (CFIR)方面的差异进行比较。结果表明 :预激态补骨脂素对K5 6 2细胞增殖有抑制作用 ;随着预激态补骨脂素浓度的增加 ,其抑制作用也随之增强 ;预激态补骨脂素与晚激态补骨脂素的TBIR、CPIR、CFIR各值比的差异不显著 ;为使预激态补骨脂素要充分发挥对K5 6 2细胞的抑制作用 ,补骨脂素的紫外线照射时间应在 10分钟以上 ;与K5 6 2细胞作用时间也应大于 12小时 ;抑制作用会因预激态补骨脂素预激后搁置时间的延长而下降 ,在 6小时内作用最强。结论 :预激态补骨脂素和晚激态补骨脂素对K5 6 2细胞的增殖均表现出抑制作用 ,有望作为临床的抗肿瘤用药。
The purpose of this study is to observe the effect of pre-excited psoralen on the proliferation of K562 cells and to provide experimental evidence for the clinical application of psoralen. Pre-excited psoralen and late-excited psoralen-treated cells were used to detect the number of trypan blue-stained cells and leukemic cell colonies after culture, and their inhibitory rate in trypan blue exclusion cells (TBIR ), Cell proliferation inhibition rate (CPIR) and colony formation inhibition rate (CFIR). The results showed that pre-excited psoralen inhibited the proliferation of K562 cells, and the inhibitory effect increased with the increase of pre-excited psoralen concentration. There was no significant difference in the ratios of TBIR, CPIR and CFIR between the excited psoralen and the psoralen. To make the pre-excited psoralen fully exert its inhibitory effect on K562 cells, the time of UV irradiation of psoralen should be In more than 10 minutes; and K562 cells should also be more than 12 hours; inhibition will be due to pre-excited psoralen pre-shock shelf-life decline and the strongest effect within 6 hours. CONCLUSION: Pre-excited psoralen and late-excited psoralen exert inhibitory effects on the proliferation of K562 cells, which is expected to be used as a clinical anti-tumor drug.