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1 材料和方法 动物分组:Wistar雄性大鼠,体重260±26g,1％戊巴比妥钠40mg/kg腹腔麻醉。随机分手术组,假手术组,正常对照组。脑出血模型制作方法参照Masuda等方法。大鼠分别在4h、12h、24h、48h、72h、120h断头处死,取出血侧兰染脑组织和对侧相应部位脑组织,测EB含量;取出血侧和对侧相应部位脑组织测LPO含量;取双侧半球测脑含水量。 脑含水量测定:采用干燥法测脑含水量,脑含水量(％)=湿重-干重)/湿重×100。脑伊文氏蓝(EB)含量测定:用分析天平精确称取标本的湿重,投入试管中分别加入3ml甲酰胺,加盖后于45℃的
1 Materials and methods Animal groups: Wistar male rats weighing 260 ± 26g, 1% sodium pentobarbital 40mg / kg intraperitoneal anesthesia. Randomly divided surgery group, sham operation group, normal control group. The method of making a cerebral hemorrhage model is based on the method of Masuda et al. The rats were sacrificed at 4h, 12h, 24h, 48h, 72h and 120h respectively. The brain tissues of the corresponding tissues in the flanks were removed and the contents of EB were measured. The contents of LPO Content; take bilateral hemisphere measured brain water content. Brain water content determination: Determination of brain water content by dry method, brain water content (%) = wet weight - dry weight) / wet weight × 100. Brain Evans Blue (EB) Determination: Accurately weighed samples with analytical balance wet weight, into the test tube were added 3ml formamide, capped at 45 ℃