论文部分内容阅读
分蘖性状不仅与玉米的进化密切相关,对玉米育种也具有重要意义.利用具有共同系谱来源的8个分蘖自交系与3个不分蘖自交系,提取DNA并分别等量混合,形成分蘖与不分蘖基因池;采用混合分离分析策略(bulked segregation analysis,BSA),利用覆盖玉米全基因组的1 013个SSR标记对2个基因池进行多态性筛选,共获得了16个与玉米分蘖相关联的位点.这16个位点分布在玉米7条染色体上,其中包括2,4,5,6,7,8和9号染色体.参照IBM2 2008高密度整合遗传连锁图谱,这16个位点分别位于11个Bin区段中,基因功能注释显示这些片段中基因的数量在7~37个之间.这些研究结果为克隆控制玉米分蘖基因奠定了良好的基础.
The tillering trait is not only closely related to the evolution of maize but also important for maize breeding.By using 8 tillering inbred lines and 3 non-tillering inbred lines with common pedigree sources, DNAs were extracted and mixed respectively to form tillering No tillering gene pool was used. By using the strategy of bulked segregation analysis (BSA), the polymorphism of two gene pools was screened by 1 013 SSR markers covering the whole genome of maize. A total of 16 maize tillers were obtained These 16 loci are distributed on 7 chromosomes of maize, including chromosomes 2, 4, 5, 6, 7, 8 and 9. Referring to the high-density integrated genetic linkage map of IBM2 2008, these 16 loci Respectively, located in 11 Bin sections, and the gene function annotation showed that the number of genes in these fragments ranged from 7 to 37. These results lay a good foundation for cloning and controlling corn tillering genes.