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目的:观察中脑黑质多巴胺(DA)神经元细胞凋亡(Programmed Cell Death,PCD)的规律。方法:选用胚胎14d的胎鼠至生后3个月的大鼠,运用酪氨酸羟化酶(TH)抗体和TUNEL免疫组化双标染色和免疫电镜法。结果:DA神经元PCD的发生始于孕16(E16),但胚胎期处于较低水平且规律不一,可能由于神经元处于增殖、分化、迁移状态的缘故。生后PCD的发生呈双峰状,第一个高峰在P2,第二个高峰在P14,高峰期恰与神经元之间发生突触整合期相吻合。结论:认为中脑黑质DA神经元PCD的发生主要于生后的早期阶段,可能与其靶器官分泌的神经营养因子的调控作用有关。
Objective: To observe the regularity of programmed cell death (PCD) of dopamine (DA) in substantia nigra of midbrain. Methods: Fetal embryos from embryonic day 14 to embryo 3 months after birth were subjected to immunohistochemical double staining and immunoelectron microscopy with tyrosine hydroxylase (TH) antibody and TUNEL. Results: The onset of PCD in DA neurons started at E16, but the embryo was at a low level with different laws. It may be due to the proliferation, differentiation and migration of neurons. The incidence of PCD after birth was bimodal, with the first peak at P2 and the second peak at P14. The peak coincided with synaptic integration between neurons. Conclusion: It is suggested that the occurrence of PCD in midbrain substantia nigra DA neurons is mainly in the early stage after birth, which may be related to the regulation of neurotrophic factors secreted by target organs.