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目的:观察石榴皮对脾虚泄泻小鼠的止泻作用并初步探讨脾虚泄泻与机体免疫功能之间的关系。方法:以清洁级昆明小鼠40只,随机分组(正常组、模型组、治疗组、对照组),采用苦寒药物灌胃致小鼠脾虚泄泻模型,治疗组以石榴皮水煎液反佐验证治疗,同时设立对照组(思密达),观察实验小鼠一般情况,以放射免疫法测定小鼠血清分泌性免疫球蛋白A(sIgA)含量等指标。结果:造模后小鼠出现活动减少、蜷卧、扎堆、摄食次数减少、大便稀溏等变化,符合脾虚泄泻证表现。治疗组小鼠症状明显改善,血清sIgA含量、T淋巴细胞百分率及转化率、腹腔吞噬细胞百分率及吞噬指数、胸腺/脾脏指数与模型组相比较(P<0.05),治疗组与对照组比较(P>0.05)。结论:石榴皮能改善脾虚泄泻小鼠症状,并能降低造模后小鼠血清sIgA含量,提高T淋巴细胞百分率及转化率、腹腔吞噬细胞百分率及吞噬指数等指标,提示石榴皮的止泻作用可能与调节机体免疫机制有关。
Objective: To observe the diarrhea effect of pomegranate peel on spleen diarrhea mice and to explore the relationship between diarrhea of spleen deficiency and immune function. Methods: Forty Kunming mice of clean grade were randomly divided into normal group, model group, treatment group and control group. The mice model of diarrhea with spleen deficiency caused by intragastric administration of bitter cold was induced. The treatment group was treated with pomegranate decoction At the same time, the control group (Smecta) was set up. The general situation of the mice was observed. The sIgA levels of the mice were determined by radioimmunoassay. Results: After modeling, the activities of mice reduced, curled up, get together, reduce the number of ingestion, stool thinning and other changes in line with spleen diarrhea evidence. Compared with the model group, the sIgA levels, the percentage of T lymphocytes and the percentage of transformation, the percentage of phagocytes in peritoneal cavity, the phagocytic index and thymus / spleen index in the treatment group were significantly improved (P <0.05) P> 0.05). CONCLUSION: Pomegranate peel can relieve the symptoms of diarrhea mice with spleen deficiency, reduce the serum sIgA level, increase the percentage and transformation rate of T lymphocytes, percentage of phagocyte in peritoneal cavity and phagocytic index, The role may be related to regulating the body’s immune system.