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Objective To prepare, characterize and evaluate the antioxidant activity of rutin-liposome(RL). Methods Liposomes of rutin were prepared by film dispersion method and the encapsulation efficiency(EE) was determined by RP-HPLC. Human umbilical vein endothelial cells(HUVECs) were injuried by H_2O_2 and treated with either free aqueous rutin or the RL delivery systems. The viability of HUVECs was determined by MTT and ELISA. Results The drug delivery system showed uniform rutin loaded nanoparticles with average particle size of(147.20 ± 1.42) nm, polydispersity index of(0.191 ± 0.003) nm, Zeta potential of(-20.0 ± 1.0) mV, and the drug EE was closed to 90.0%. The antioxidant effect of the drug delivery system to H_2O_2-damaged HUVECs showed that RL could increase injury cells viability compared to free aqueous rutin, which was accompanied with an obvious decrease in malondialdehyde(MDA), lactate dehydrogenase(LDH) while increase the level of nitrogen oxide(NOS). Conclusion The nanostructured RL is improved on the antioxidant effect and may be treating the different diseases caused by free radicals.
Objective To prepare, characterize and evaluate the antioxidant activity of rutin-liposome (RL). Methods Liposomes of rutin were prepared by film dispersion method and the encapsulation efficiency (EE) was determined by RP-HPLC. Human umbilical vein endothelial cells (HUVECs) The injunction of HUVECs was determined by MTT and ELISA. The drug delivery system showed uniform rutin loaded nanoparticles with average particle size of (147.20 ± 1.42) nm, The polydispersity index of (0.191 ± 0.003) nm, Zeta potential of (-20.0 ± 1.0) mV, and the drug EE was closed to 90.0%. The antioxidant effect of the drug delivery system to H_2O_2-damaged HUVECs showed that RL could increase injury cells viability compared to free aqueous rutin, which was accompanied with an obvious decrease in malondialdehyde (MDA), lactate dehydrogenase (LDH) while increase the level of nitrogen oxide (NOS). Conclusion The nanostru ctured RL is improved on the antioxidant effect and may be treating the different diseases caused by free radicals.