论文部分内容阅读
目的 探讨反复呼吸道感染（ ＲＲＴＩ） 患儿淋巴细胞免疫功能的动态变化， 方法 采用ＬＴＴ，间接免疫荧光法，ＥＬＩＳＡ 等方法，对５４ 例ＲＲＴＩ 患儿和２１ 例对照组儿童的外周血淋巴细胞的增殖应答效应，ＩＬ２ Ｒ 的表达能力，Ｔ 细胞亚群，ＣＤ４／ＣＤ８ 比值等项目进行了检测。 结果 ＲＲＴＩ 患儿与对照儿童的ＰＢＬ 增殖应答能力无显著差异（ Ｐ＞ ０ ．０５） ；Ｔ 细胞亚群，ＣＤ４／ ＣＤ８ 比值有显著差异（ Ｐ＜ ０ ．０１） ；ＲＲＴＩ 患儿的活化淋巴细胞ＩＬ２Ｒ 表达下降，血浆中和细胞培养上清液中ＳＩＬ２Ｒ 水平升高。 结论 ＲＲＴＩ 患儿细胞免疫功能低下，ＣＤ４／ ＣＤ８ 比值异常，影响体液免疫，主要是分泌型ＩｇＡ 的产量降低，使患儿的局部防御功能降低，导致细菌、病毒的反复感染。
Objective To investigate the dynamic changes of lymphocyte immune function in children with recurrent respiratory tract infection (RRTI). Methods LTT, indirect immunofluorescence and ELISA were used to detect the proliferation of peripheral lymphocytes in 54 RRTI children and 21 control children Response effect, the expression of IL 2 R, T cell subsets, CD4 / CD8 ratio and other items were tested. Results There was no significant difference in PBL proliferative response between children with RRTI and control children (P> 0.05). There was significant difference in T cell subsets and CD4 / CD8 ratio (P <0.01). The percentage of activated lymphocytes IL 2R expression decreased plasma and cell culture supernatant SIL 2R levels. Conclusions Children with RRTI have poor cellular immunity and abnormal CD4 / CD8 ratio, which may affect humoral immunity. The production of secreted IgA is reduced, which leads to the reduction of partial defensive function in children and the repeated infection of bacteria and viruses.