目的：观察蝎毒抗癌多肽（ａｎｔｉｎｅｏｐｌａｓｔｉｃｐｏｌｙｐｅｐｔｉｄｅｆｒｏｍＢｕｔｈｕｓＭａｔｅｎｓｉｖｅｎｏｍ，ＡＰＢＭＶ）对人肿瘤细胞生长的抑制作用。方法：采用噻唑蓝（ＭＴＴ）法、台盼蓝拒染法和集落形成观察为指标，丝裂霉素Ｃ（ＭＭＣ）为阳性对照药品，以ＡＰＢＭＶ４ｍｇ／Ｌ，８ｍｇ／Ｌ，１２ｍｇ／Ｌ和１６ｍｇ／Ｌ处理４种人肿瘤细胞（人早幼粒白血病细胞株ＨＬ６０、人肝细胞癌细胞株ＳＭＭＣ７７２１、人低分化鼻咽上皮癌细胞株ＣＮＥ２Ｚ和人胃癌细胞株ＭＧＣ８０３），选用不同的作用时间点，分别观察ＡＰＢＭＶ对上述细胞的影响。结果：ＡＰＢＭＶ对ＨＬ６０、ＳＭＭＣ７７２１、ＣＮＥ２Ｚ和ＭＧＣ８０３细胞均有显著的细胞毒性作用，ＨＬ６０和ＳＭＭＣ７７２１比后两者显示出更好的量效关系（ｒ＝０．９８２８，ｒ＝０．９８６３，Ｐ＝０．０２）。ＡＰＢＭＶ处理ＨＬ６０和ＳＭＭＣ７７２１细胞２４ｈ，４８ｈ和９６ｈ后，两种细胞的生长均受到抑制（Ｐ＜０．０５，Ｐ＜０．０１），并有显著的剂量效应关系；处理ＳＭＭＣ７７２１细胞２４ｈ，４８ｈ和９６ｈ时ＡＰＢＭＶ的ＩＣ５０分别是１５．８７ｍｇ／Ｌ、１３．０５ｍｇ／? OBJECTIVE: To observe the inhibitory effect of antineoplasticpolypeptide from Brugus Matanii (APBMV) on the growth of human tumor cells. Methods: MTT assay, trypan blue exclusion assay and colony formation assay were used as indexes. Mitomycin C (MMC) was used as positive control drug. APBMV4mg / L, 8mg / L, 12mg / L And 16mg / L treatment of four human tumor cells (human promyelocytic leukemia cell line HL-60, human hepatocellular carcinoma cell line SMMC 7721, human poorly differentiated nasopharyngeal epithelial cancer cell line CNE 2Z and human gastric cancer cell line MGC  803), select the different time points, were observed APBMV impact on the cells. Results: APBMV had significant cytotoxic effect on HL60, SMMC7721, CNE2Z and MGC803 cells, and HL60 and SMMC7721 showed better dose-response relationship than the latter two (r = 0.9828, r = 0.9863, P = 0.02). APBMV treatment of HL  60 and SMMC  7721 cells 24h, 48h and 96h, the growth of both cell lines were inhibited (P <0.05, P <0.01), and a significant dose-response relationship; SMMC  treatment The IC50 of APBMV in 7721 cells at 24h, 48h and 96h were 15.87mg / L and 13.05mg /