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目的探讨口服抗生素是否引起大鼠结肠炎症以及合成去甲肾上腺素(Norepinephrine,NE)的酶即多巴胺β羟化酶(Dopamine-β-hydroxylase,Dβh)在结肠中的变化。方法在雄性SD大鼠的饮用水中添加肠道不吸收的抗生素(新霉素、杆菌肽和游霉素),饮用1周、3周之后分别取结肠组织进行组织病理学观察,并用实时定量PCR检测大鼠结肠促炎因子白细胞介素-1β(Interleukin-1β,IL-1β)和抗炎因子白细胞介素-10(Interleukin-10,IL-10)以及Dβh mRNA的表达水平。结果与对照组相比,饮用抗生素1周大鼠结肠HE(Haematoxylin and eosin)染色显示轻微的炎症,3周时结肠炎症略有加重;饮用抗生素1周、3周大鼠结肠IL-1β的mRNA水平上升(P<0.01),IL-10的mRNA水平下降(P<0.01),而Dβh的mRNA表达在1周时,与对照组比较差异无统计学意义,3周时显著升高(P<0.05)。结论大鼠口服抗生素可引起其结肠的轻度炎症并且结肠Dβh的表达在3周时显著升高。
OBJECTIVE: To investigate whether oral antibiotics cause colitis in the colon and changes in the colon of Dopamine-β-hydroxylase (Dβh), an enzyme that synthesizes norepinephrine (NE). Methods The intestinal non-absorbed antibiotics (neomycin, bacitracin and natamycin) were added to the drinking water of male SD rats for one week. After three weeks, the colon tissues were taken for histopathological observation and quantified by real-time quantitative PCR was used to detect the expression of Interleukin-1β (IL-1β) and interleukin-10 (IL-10) and Dβh mRNA in rats. Results Compared with the control group, colon cancer HE (Haematoxylin and eosin) stained with antibiotics for 1 week showed slight inflammation, and inflammation of the colon slightly increased after 3 weeks. The levels of IL-1β (P <0.01). The mRNA expression of Dβh at 1 week was not significantly different from that of the control group (P <0.01), but significantly increased at 3 weeks (P < 0.05). Conclusion Oral antibiotics in rats can cause mild inflammation in the colon and the expression of Dβh in colon increased significantly at 3 weeks.