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目的研究3,5,2’,4’-四羟基查尔酮(P40)对高尿酸血症大鼠尿酸的影响及对PC12细胞嘌呤代谢关键酶的影响.方法灌胃给予SD大鼠P40(2.0、4.0和8.0 mg/kg)5次,末次给药前1 h腹腔注射氧嗪酸钾(250mg/kg)诱导成高尿酸血症动物模型,注射后2 h取血,用磷钨酸法测定血尿酸水平及肝尿酸含量.给予PC12细胞系列浓度的P40和阳性对照药别嘌醇后培养48 h,收集细胞提取总RNA,采用逆转录聚合酶链(RT-PCR)的方法,检测嘌呤代谢关键酶HGPRT、PRPS和PRPPAT m RNA的表达水平.结果给予P40 2.0、4.0 mg/kg均能显著降低高尿酸血症大鼠的血尿酸水平,和模型组相比,差异有统计学意义(P<0.01),但对肝尿酸含量没有影响.给予P40 1×10~(-8),1×10~(-7),1×10~(-6) mol/L处理PC12细胞48 h后,对HGPRT、PRPS和PRPPAT基因的m RNA表达没有明显影响,与对照组相比差异无统计学意义(P<0.01).结论在本实验条件下,P40能降低氧嗪酸钾诱导的高尿酸血症大鼠的血尿酸水平,对PC12细胞的HGPRT、PRPS和PRPPAT基因的m RNA表达无影响.
Objective To study the effects of 3,5,2 ’, 4’-tetrahydroxychalcones (P40) on uric acid in hyperuricemic rats and the key enzymes of purine metabolism in PC12 cells.Methods The rats were administered orally with P40 2.0, 4.0 and 8.0 mg / kg) five times. One hour before the last administration, oxonic acid potassium (250mg / kg) was injected into the animal model of hyperuricemia. The level of serum uric acid and the content of hepatic uric acid were determined.P40 and PC12 cells were treated with allopurinol for 48 h and the total RNA was collected.The expression of purine was detected by reverse transcriptase polymerase chain reaction (RT-PCR) Metabolism of key enzymes HGPRT, PRPS and PRPPAT m RNA expression levels.Results P40 2.0,4.0 mg / kg can significantly reduce hyperuricemia in rats with uric acid levels, compared with the model group, the difference was statistically significant ( P <0.01), but had no effect on the content of hepatic uric acid.The PC12 cells were treated with P40 1 × 10 ~ (-8), 1 × 10 ~ (-7) and 1 × 10 ~ (-6) mol / L for 48 h , No significant effect on mRNA expression of HGPRT, PRPS and PRPPAT, and no significant difference compared with the control group (P <0.01) .Conclusion Under the experimental conditions, P40 can reduce potassium oxonic acid-induced hyperuricemia blood The level of serum uric acid in the diseased rat had no effect on the mRNA expression of HGPRT, PRPS and PRPPAT in PC12 cells.