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目的探讨磺胺间甲氧嘧啶(SMM)哺乳期暴露对青春期小鼠探索和焦虑行为影响。方法小鼠哺乳期以0(对照组)、10、50、200 mg/kg灌胃染毒,连续21 d,断乳后其仔鼠雌、雄分笼正常喂养至49 d,次日进行旷场、黑白巷和高架十字迷宫实验。结果与对照组雄鼠比较,低、中剂量SMM组雄鼠旷场实验中央区域时间[分别为(40.6±10.0)、(41.0±11.9)s]增加,差异有统计学意义(P<0.05),中剂量组雄鼠站立次数[(56.5±8.8)次]明显增加,差异有统计学意义(P<0.01);与对照组雌鼠比较,低剂量SMM组雌鼠旷场实验中央区域时间[(37.3±9.0)s]增加,差异有统计学意义(P<0.05),中剂量SMM组雌鼠的跨越格子数[(159.1±26.0)个]增加,差异有统计学意义(P<0.05);高剂量SMM组雌鼠黑白巷实验黑端时间[(57.8±8.2)s]增加,差异有统计学意义(P<0.01)。结论哺乳期SMM暴露可干扰仔鼠的探索和焦虑等行为。
Objective To investigate the effects of sulfamonomethoxine (SMM) during lactation on the exploration and anxiety behavior in adolescent mice. Methods Mice were orally gavaged with 0 (control group), 10,50,200 mg / kg for 21 days. After lactation, the female and male offspring rats were fed normal diet for 49 d. The next day, Field, Black and White Alley and elevated maze experiment. Results Compared with the male rats in the control group, the median open field time ([(40.6 ± 10.0) and (41.0 ± 11.9) s] in open field test in male and female SMM groups were significantly increased (P <0.05) (56.5 ± 8.8) times, the difference was statistically significant (P <0.01). Compared with the female rats in the control group, the open field test time of the female rats in the low-dose SMM group was significantly higher than that of the control group (37.3 ± 9.0) s], the difference was statistically significant (P <0.05). The number of crossed squares in middle dose SMM group [(159.1 ± 26.0)] was increased, the difference was statistically significant (P0.05) ; The black end time of black-white alley in high-dose SMM group [(57.8 ± 8.2) s] increased, the difference was statistically significant (P <0.01). Conclusion Exposure to lactating SMM can interfere with the exploration and anxiety behavior of offspring.