在研究噬菌体预测水稻白叶枯病发生的工作中,首先必须分离出当地的白叶枯病病原菌作为指示菌,但在1974年以前尚未找到比较合适的分离培养方法。我们于当年进行了这方面的试验,并取得初步结果。一、试验方法(一)分离培养1、培养基配方味精培养基:食用味精2克、硫酸亚铁1毫克、蔗糖5克、氧化镁1克、磷酸氢二钾1克、蛋白胨10克、琼脂20克、水1000毫升,pH7—8。 In the study of bacteriophage prediction of rice bacterial leaf blight, we must first isolate the local bacterial blight pathogen as indicator bacteria, but in 1974 has not been found more appropriate isolation and culture methods. In the same year, we conducted experiments in this area and achieved initial results. First, the test method (a) separation and culture 1, the medium formula MSG medium: MSG 2 g, ferrous sulfate 1 mg, sucrose 5 g, magnesium oxide 1 g, 1 g of dipotassium hydrogen phosphate, peptone 10 g, agar 20 grams, 1000 milliliters of water, pH 7-8.