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目的对2009年发生在安徽省境内的2起霍乱疫情进行溯源及2起疫情菌株的遗传相关性分析,为防控霍乱提供依据。方法应用血清学及常规细菌学方法对菌株进行鉴定,聚合酶链式反应(PCR)方法检测霍乱毒力基因,脉冲场凝胶电泳技术(PFGE)对霍乱菌株进行分子分型。结果所分离3株菌均是霍乱弧菌O139且具有肠毒素(CT)和毒素共调菌毛(TCP)毒力基因;PFGE分成2个型。SZ分离株的pattern在福建、广东、湖南、江浙和重庆出现过。该pattern的菌株在霍乱数据库占已有O139菌株的12.7%;。H1、H2的pattern与辽宁分离株具有相同的pattern。该pattern的菌株在霍乱数据库中目前仅有1株。结论 3株试验菌株均是产毒株,具有致病性;首例病人分离株与该病人所食甲鱼分离株PFGE带型一致,分子流行病学分析,甲鱼可能是首起疫情的传染源之一,与第二起疫情无相关性。
Objective To trace the origin of 2 cholera epidemics in Anhui province and the genetic correlation analysis of 2 epidemic strains in 2009 so as to provide basis for prevention and control of cholera. Methods Serological and routine bacteriological methods were used to identify the strains. Polymerase chain reaction (PCR) was used to detect the cholera virulence genes. The genotypes of cholera were identified by pulsed-field gel electrophoresis (PFGE). Results All three strains isolated were Vibrio cholerae O139 and had enterotoxin (CT) and toxin-co-stimulated pilus (TCP) virulence genes; PFGE was divided into two types. The pattern of SZ isolates appeared in Fujian, Guangdong, Hunan, Jiangsu, Zhejiang and Chongqing. The pattern of the strain accounts for 12.7% of the existing O139 strain in the cholera database; H1, H2 pattern and Liaoning isolates have the same pattern. The pattern of the strain in the cholera database is currently only 1 strain. CONCLUSION: The three strains were all virulent and virulent. The isolates from the first patient were consistent with the PFGE patterns of the isolates of the soft-shelled turtle. The molecular epidemiological analysis indicated that the turtle may be the source of the first outbreak First, there is no correlation with the second epidemic.