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目的:探讨葡萄籽原花青素(grape seed proanthocyanidin extract,GSPE)对大鼠烟雾吸入性肺损伤的保护作用。方法:将48只大鼠随机分为正常对照组、烟雾吸入性肺损伤模型组、GSPE治疗组(500mg/kg),分别于致伤后2、4、12、24h监测动脉血气分析,分批处死大鼠,分别进行肺组织湿/干重测定,制备组织匀浆测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮合酶(NOS)活性及一氧化氮(NO)、丙二醛(MDA)含量和HE染色。结果:与模型组比较,GSPE治疗组各时间点动脉血氧分压均显著升高(P<0.01),肺组织含水量显著降低(P<0.05),肺组织中SOD活性均明显升高(P<0.01),GSH-Px活性均明显升高(P<0.05),NOS活性及NO、MDA含量均明显降低(P<0.05)。肺组织病理学观察GSPE治疗组较模型组肺间质水肿减轻,炎性细胞浸润减少。结论:GSPE可能通过其显著增加组织的抗氧化能力而对烟雾吸入性肺损伤起到一定保护作用。
Objective: To investigate the protective effect of grape seed proanthocyanidin extract (GSPE) on smoke-induced lung injury in rats. Methods: Forty-eight rats were randomly divided into normal control group, smoke inhalation lung injury model group and GSPE treatment group (500mg / kg). Arterial blood gas analysis was performed at 2,4,12,24h after injury, The rats were sacrificed and the wet / dry weight of lung tissue was determined respectively. The content of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nitric oxide synthase (NOS) Activity and nitric oxide (NO), malondialdehyde (MDA) content and HE staining. Results: Compared with model group, the partial pressure of arterial oxygen of GSPE treatment group increased significantly (P <0.01), the water content of lung tissue decreased significantly (P <0.05), the activity of SOD in lung tissue increased significantly P <0.01). The activities of GSH-Px were significantly increased (P <0.05) and the activity of NOS and contents of NO and MDA were significantly decreased (P <0.05). Lung histopathological observation GSPE treatment group than the model group, interstitial edema, inflammatory cell infiltration decreased. Conclusion: GSPE may play a protective role in smoke-induced lung injury through its significant increase in the antioxidant capacity of tissues.