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目的 :观察体外培养的鼠磨牙牙胚发育过程的组织学变化。方法 :用 16d胎龄的昆明小鼠下颌第一磨牙牙胚置于RPMI- 16 40半固态培养基上培养 ,常规组织学观察。结果 :体外培养的牙胚可由帽状期进入钟状期 ,出现成牙本质细胞和成釉细胞的分化及前期牙本质的形成。结论 :半固态培养基法培养牙胚可保持其发育过程。
Objective: To observe the histological changes of mouse molar tooth germ during in vitro culture. Methods: The mandibular first molar tooth germ of 16-day-old Kunming mice was placed on RPMI-1640 semi-solid medium and cultured for routine histological examination. Results: The tooth germ cultured in vitro could enter into the bell phase from the cap-like phase. The differentiation of odontoblasts and ameloblasts and the formation of pre-dentin appeared. Conclusion: Semi-solid medium culture of tooth germ can keep its development.