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魔芋是天南星科多年生草本植物。其传统的繁殖方法是种植地下球茎,繁殖系数低,产品质量较差。我们从1985年4月开始了魔芋的组培工作,目前已成功地诱导出愈伤组织,并得到了再生植株。取湖北农家品种兴山魔芋的幼嫩芽鞘作外植体,经0.1%升汞消毒,无菌水冲洗后,接种在附加 BA 和 NAA 各0.6—1.5mg/1(单位下同),的 MS 培养基上,培养3—4周,形成粉红色的愈伤组织,将愈伤组织及时转移到附加BA4、IBA 0.25的 MS 分化培养基中,经过2周出现许多绿色小芽点,四周以后少数芽点分化出粗壮的锥形芽,外包暗红带褐色斑点的芽鞘,
Konjac is Araceae perennial herb. Its traditional breeding method is to grow underground bulbs, with low propagation coefficient and poor product quality. We started the tissue culture of Konjak in April 1985 and have succeeded in inducing callus and regenerating plants. The young shoots of Amorphophallus konjac from Hubei were taken as explants and sterilized with 0.1% mercuric chloride and rinsed with sterile BA and NAA respectively for 0.6-1.5mg / 1 (the same below) MS medium for 3-4 weeks to form a pink callus, the callus was promptly transferred to additional BA4, IBA 0.25 MS differentiation medium, after 2 weeks there are many small green shoots, four weeks later A small number of bud points out of the thick conical buds, dark brown outsourcing dark spots bud sheath,