论文部分内容阅读
目的:研究猪脾脏中的一种具有免疫抑制活性的蛋白质,纯化该蛋白并研究其与临床疾病的关系。方法:1)稀酸提取;2)超滤透析分离分子量大于M_r30 000的分子;3)FPLC免疫亲和层析纯化;4)T淋巴细胞转化及ELISA检测其功能活性;5)SDS-PAGE检测其分子量及纯度。结果:该蛋白能抑制由Con A诱导的淋巴细胞增殖。其分子量在190 000 左右。该蛋白对某些来源于免疫细胞系的细胞,具有明显的选择性抑制作用。结论:成功部分纯化这种蛋白,并证明其与应激免疫抑制蛋白有相似的理化、免疫原性和功能活性。这一研究为进一步大量纯化该蛋白提供了实用和稳定的生物学来源,并为研究它与肿瘤、自身免疫性等临床疾病关系开辟了一条新的研究途径。
Objective: To study a protein with immunosuppressive activity in porcine spleen, purify the protein and study its relationship with clinical disease. Methods: 1) Diluted acid extraction; 2) ultrafiltration dialysis to separate molecules with molecular weight greater than M_r30 000; 3) purification by FPLC immunoaffinity chromatography; 4) T lymphocyte transformation and ELISA to test its functional activity; 5) SDS- Its molecular weight and purity. Results: The protein inhibits Con A-induced lymphocyte proliferation. Its molecular weight is around 190,000. The protein has obvious selective inhibitory effect on some cells derived from immune cell line. Conclusion: The partial purification of this protein was successful and proved to be similar in physicochemical, immunogenicity and functional activity to stress immunosuppressive proteins. This study provides a practical and stable biological source for further purification of the protein in large quantities and opens up a new research approach for studying its relationship with clinical diseases such as tumor and autoimmune diseases.