一、菌种的培养基物。供食菌菌种分离培养的基物很多,概括起来有3种:完全培养基——能满足任何微生物的营养需要,其成份都是已知的如察氏培养基,马丁培养基等;基本培养基——由天然原料与部分已知有机物合成如马铃薯·葡萄糖琼脂培养基(简称PDA)、木汁。米糠琼脂培养基等;补充培养基——菌株经诱变后不能在基本培养基上生长,必须在培养基中补充某种有机营养成分。目前分离香菇,平菇(冻菌)、猴头,木耳,银耳都习惯用PDA培养基。一般地说只要配方准确,操作认真细致,菌丝体都能良好生长。腐生菌分解木质纤维素的能力很强,在许多腐朽的木桩上子实体往往生长发育得格外健壮。根据这一特性我们从1974年起用木汁(谷草,麦草,玉米芯即可)米糠琼脂培养 First, the bacteria culture medium. There are a lot of substrates for the isolation and culture of the bacteria for feeding bacteria, which can be summed up in three ways: Complete medium - can meet the nutritional needs of any microorganism and its components are known such as Czochralszki and Martin medium; Medium - Synthesized from natural raw materials and partially known organics such as potato dextrose agar (PDA), wood juice. Rice bran agar medium, etc .; supplemented medium - Strain mutagenesis can not grow on the basic medium, the medium must be supplemented with some organic nutrients. Currently separated mushrooms, mushroom (frozen), Hericium, fungus, white fungus are accustomed to using PDA medium. Generally speaking, as long as the formula is accurate and careful operation, mycelium can grow well. The ability of saprophytic bacteria to decompose lignocellulose is very strong, and in many decayed wooden piles, the fruiting bodies tend to grow and develop exceptionally robustly. According to this feature, we have used wood juice (straw, wheat straw and corn cob) from 1974 to cultivate rice bran agar