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目的:研究黄杨宁(cyclovirobuxine D,CVB-D)对大鼠胸主动脉的舒张作用,并探讨其可能的作用机制。方法:分别采用氯化钾(KCl)和去氧肾上腺素(PE)预收缩血管,观察CVB-D(1×10-5~6×10-4mol·L-1)对血管的舒张作用;将血管与6×10-4mol·L-1CVB-D预孵育,观察其对KCl或PE收缩血管作用的影响;观察不同抑制剂对CVB-D舒张大鼠离体血管环作用的影响。结果:在给药浓度范围内,CVB-D对KCl或PE预收缩血管环的舒张作用呈剂量依赖性;CVB-D对内皮完整血管环的舒张作用强于对去内皮血管环的舒张作用;CVB-D与血管预孵育可抑制KCl或PE引起的血管收缩;一氧化氮合成酶(NOS)抑制剂左旋硝基精氨酸甲酯(L-NAME)或选择性一氧化氮(NO)敏感的可溶性鸟苷酸环化酶(sGC)抑制剂ODQ可阻断CVB-D的血管舒张作用。结论:CVB-D对大鼠离体胸主动脉的血管舒张作用呈剂量依赖性,其舒张作用可能与一氧化氮-可溶性鸟苷酸环化酶-环磷酸鸟苷(NO-sGC-cGMP)途径相关。
Objective: To study the vasodilatory effect of cyclovirobuxine D (CVB-D) on the thoracic aorta in rats and its possible mechanism. Methods: Premature contraction of blood vessels by potassium chloride (KCl) and phenylephrine (PE) was used to observe the vasodilatation effect of CVB-D (1 × 10-5 ~ 6 × 10-4 mol·L-1) Blood vessels were pre-incubated with 6 × 10-4mol·L-1CVB-D to observe the effect of KCl or PE on vasoconstriction. The effects of different inhibitors on the isolated vascular rings in CVB-D relaxation rats were observed. Results: In the concentration range of administration, CVB-D had a dose-dependent effect on the relaxation of KCl or PE pre-contracted vascular rings; the diastolic effect of CVB-D on intact endothelial rings was stronger than that of the endothelium- CVB-D pre-incubation with blood vessels can inhibit the vasoconstriction induced by KCl or PE; the nitric oxide synthase (NOS) inhibitor L-NAME or NO sensitive ODG, a soluble guanylate cyclase (sGC) inhibitor, blocked the vasorelaxation of CVB-D. Conclusions: The vasodilatation of rat isolated thoracic aorta in a dose-dependent manner may be related to the vasodilatation of CVB-D, which may be related to nitric oxide-soluble guanylate cyclase-NO-sGC-cGMP Pathways related.