[Objective] To establish methods for determining contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing,so as to provide a scientific basis for further development and utilization of this resources and quality control.[Method] Contents of total flavonoids and rutin were respectively determined by aluminium trichloride colorimetric method and HPLC.[Result] Contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing were 5.485-5.742 and 0.769-0.811 mg/g,respectively.[Conclusion] The method was simple,rapid,accurate,stable and suitable for determining the content of total flavonoids and rutin in Ephorbia helioscopia L.. [Objective] To establish methods for determining contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing, so as to provide a scientific basis for further development and utilization of this resources and quality control. [Method] Contents of total flavonoids and rutin were evaluated by aluminum trichloride colorimetric method and HPLC. [Result] Contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing were 5.485-5.742 and 0.769-0.811 mg / g, respectively. [Conclusion] The method was simple, rapid , accurate, stable and suitable for determining the content of total flavonoids and rutin in Ephorbia helioscopia L ..