目的检测严重生精功能障碍患者外周血染色体,为拟行ICSI技术助孕的患者提供遗传咨询。方法按常规方法进行外周血淋巴细胞培养、染色体制备、核型分析。结果61例无精子症患者中有22例染色体核型异常,异常率36.1%;36例严重少精子症患者中有1例染色体异常,异常率2.78%,根据染色体结果及生精功能障碍程度选择不同的诊治方案。结论(1)染色体异常是导致男性生精功能障碍的重要原因之一;(2)ICSI助孕前,夫妇双方须行遗传学检查以避免遗传缺陷后代的出生。 Objective To detect the chromosomes of peripheral blood in patients with severe spermatogenic dysfunction and provide genetic counseling for patients who intend to give birth by ICSI. Methods Peripheral blood lymphocyte culture, chromosome preparation and karyotype analysis were performed according to the routine methods. Results Twenty-one patients with azoospermia had abnormal karyotype and abnormal rate of 36.1%. One of 36 severe oligospermia patients had chromosomal abnormalities with an abnormality rate of 2.78%. According to the chromosomal results and the degree of dysfunctional spermatogenesis, Different treatment programs. Conclusions (1) Chromosomal abnormalities are one of the most important causes of dysfunctional spermatogenesis in men. (2) Before ICSI pregnancy, both husband and wife need to carry out genetic tests to avoid the birth of the genetic defective offspring.