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本文应用Fourier变换红外光谱法(FTIR)分别探测膜脂质双层碳氢链尾部区、脂相-水相交界区和头部区的物理状态,以了解没食子酸及其酯类(没食子酸乙酯、丙酯、异丁酯、丁酯)的作用机制。结果表明,没食子酸及其酯类都有调节DPPC人工膜和含胆固醇的DPPC人工膜的作用,并呈现量效关系和构效关系。还首次发现,上述酯类也对人血小板膜的结构有调节作用,而且可以使ADP作用后的血小板膜发生可逆性恢复。该类化合物可抵消胆固醇对人工膜和ADP对血小板的作用这一现象,为解释活血化瘀中药作用机制提供一些新的论据。
In this paper, Fourier transform infrared spectroscopy (FTIR) was used to detect the physical state of the membrane lipid double-stranded tail, the lipid-water junction and the head region, respectively, to understand the effects of gallic acid and its esters Ester, propyl ester, isobutyl ester, butyl ester) mechanism of action. The results showed that gallic acid and its esters could regulate the DPPC artificial membrane and the cholesterol-containing DPPC artificial membrane, and showed a dose-effect relationship and a structure-activity relationship. It is also found for the first time that these esters also have a regulatory effect on the structure of human platelet membranes and can reversibly restore the platelet membrane after ADP action. The compounds can counteract the effect of cholesterol on artificial membranes and the effect of ADP on platelets, and provide some new arguments for explaining the mechanism of action of traditional Chinese medicine on promoting blood circulation and removing blood stasis.