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本文采用抗原捕捉ＥＬＩＳＡ方法检测了ＨＣＶ感染者血清中抗－ＨＣＶＩｇＧ抗体轻链κ和λ的比值，发现所检测的抗ＨＣＶ－ＮＳ４、抗ＨＣＶ－ＣＰ１和抗ＨＣＶ－ＣＰ２抗体轻链的表达呈现明显的偏斜，６５例抗ＨＣＶ阳性者中６３例（占９６．９％），至少一种抗ＨＣＶ抗体κ／λ偏离了正常１：１的比值，尤以λ链较多，分别占６５．６％、８９．９％和７０．２％，但任何一个ＨＣＶ感染者血清抗－ＨＣＶ抗体既可能是κ链占优势，也可能是λ链为主；对６例抗ＨＣＶ阳性者追踪观察了一年，发现抗ＨＣＶ抗体κ／λ比值稳定不变。提示感染ＨＣＶ后，病毒抗原可能稳定地刺激单个或少数Ｂ细胞优势克隆产生抗－ＨＣＶ抗体现为ＨＣＶ感染者抗－ＨＣＶ抗体产生的不均匀性和Ｂ细胞优势克隆的存在，这一结果将有助于深入研究宿主抗ＨＣＶ应答的规律，为抗ＨＣＶ保护性免疫与ＨＣ疫苗研究提供线索。
In this study, antigen-capture ELISA was used to detect the ratio of the light chain κ and λ of anti-HCV antibody in HCV-infected sera. The results showed that the light chain of anti-HCV-NS4, anti-HCV-CP1 and anti-HCV- (65.9%) of 65 anti-HCV positive patients. The ratio of at least one anti-HCV antibody κ / λ deviated from the normal ratio of 1: 1, especially in the case of λ chain, accounting for 65%. 6%, 89.9% and 70.2%, respectively. However, the serum anti-HCV antibodies in any HCV-infected patients were either predominantly κ or predominantly λ-chains. Six anti-HCV positives were followed up One year, the anti-HCV antibody κ / λ ratio was found to be stable. Suggesting that viral antigens may stably stimulate the production of anti-HCV antibodies by dominant clones of single or a few B-cells after infection with HCV is presently present as an inhomogeneity of anti-HCV antibodies in HCV-infected individuals and in the presence of predominantly B-cell clones, which will have Which will help to further study the host anti-HCV response rules and provide clues for the study of HCV protective immunity and HC vaccine.