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用Langendorff离体心脏灌流法研究了苯乙阱对59只豚鼠和16只猫的冠脉流量、心搏率和心縮力的作用,并探討它与儿茶酚胺和5-羟基色胺(5-HT)的关系.(1)苯乙肼10~(-6)M对豚鼠搏动心脏有明显抑制,同时增加冠脉流量(+36％),其增加程度比在顫动心脏(+8％)更为明显,說明对搏动心脏增加冠脉流量的3/4是由于抑制心縮力而降低冠脉外阻力,另1/4是直接扩张冠脉的結果.对离体猫心的心搏率和心縮力无明显影响,因此在搏动和顫动心脏增加冠脉流量的程度近似(分別为+8％和+9％).(2)苯乙肼10~(-6)M灌流正常豚鼠心脏不但能消除脑垂体后叶素(0.5单位/升)減少冠脉流量(-23％)的作用,且使冠脉流量增加(+18％).預先腹腔注射苯乙肼(15毫克/公斤/天)連續6天的“苯乙肼化”豚鼠心脏使脑垂体后叶素收縮冠脉的反应基本消失.(3)苯乙肼对“利血平化”(經酪胺証实心脏內儿茶酚胺已被耗竭)的豚鼠和猫心的作用与对正常心脏的作用无明显差別,提示它的作用与释放儿茶酚胺关系較小.(4)“利血平化”的豚鼠心脏对5-HT的反应比正常者敏感.“苯乙肼化”和苯乙阱一次灌流后的豚鼠心脏对5-HT的反应均无显著加强,表明苯乙肼大概不是通过增加5-HT而扩张冠脉的.
The effect of phenylbutyrate on coronary flow, heart rate and cardiac contractility in 59 guinea pigs and 16 cats was investigated by Langendorff perfusion in vitro and its relationship with catecholamine and 5-hydroxytryptamine (5-HT (1) phenelzine 10 ~ (-6) M significantly inhibited the pulsating heart of guinea pigs and increased the coronary flow (+ 36%) more than the fibrillation heart (+ 8%) Is obvious, indicating that pulsatile heart increased coronary flow 3/4 is due to inhibition of cardiac contractility and reduce the extracorporeal resistance, and the other quarter is the direct expansion of coronary artery results on isolated cat heart rate and (2) Phenylhydrazine 10 ~ (-6) M Perfused normal heart of guinea pigs (0.5 mg / kg / d) reduced coronary flow (-23%) and increased coronary flow (+ 18%). Day) for 6 consecutive days “phenelzyl” guinea pig heart pituitrin vasoconstrictor coronary response basically disappeared. (3) phenelzine “reserpine” (confirmed by tyramine in the heart of catecholamines Have been depleted) of the dolphin The role of the heart of rats and cats and the role of normal heart no significant difference, suggesting that its role and release of catecholamines less. (4) “reserpine” guinea pig heart response to 5-HT than the normal sensitivity. There was no significant increase in response to 5-HT in phenelzine and pethidine well once perfused, suggesting that phenelzine did not dilate coronary arteries by increasing 5-HT.