目的建立石墨炉原子吸收光谱测定全血及尿液中铍的方法。方法石墨管经氧氯化锆镀层后,用硝酸镧作基体化学改进剂,全血样品以硝酸和TrittonX-100为样品直接稀释液,尿液以1%硝酸稀释,经优化条件后,利用石墨炉原子吸收光谱仪自动进样测定。结果此方法的线性范围为0.00μg/L~5.00μg/L,全血样品的相关系数为0.9993,尿液样品的相关系数为0.9999,连续6 d测定的相对标准偏差(RSD)全血为4.52%~5.75%,尿液为3.16%~6.86%,加标回收率全血为97.1%~109.4%,尿液为98.6%~106.0%。结论本方法灵敏度高,重复性好,结果准确可靠,自动化程度高,操作简便,适合于批量测定从业人员体内铍的浓度。 Objective To establish a method for the determination of beryllium in whole blood and urine by graphite furnace atomic absorption spectrometry. Methods After the graphite tube was coated with oxychloride, the lanthanum nitrate was used as the matrix chemical modifier. The whole blood samples were diluted directly with nitric acid and Tritton X-100, and the urine was diluted with 1% nitric acid. After optimizing the conditions, graphite Furnace Atomic Absorption Spectrometer Automatic Injectio. Results The linear range of this method was 0.00μg / L ~ 5.00μg / L, the correlation coefficient of whole blood sample was 0.9993, the correlation coefficient of urine sample was 0.9999, the relative standard deviation (RSD) whole blood was 4.52 % To 5.75%, urine from 3.16% to 6.86%, spiked recoveries of whole blood from 97.1% to 109.4% and urine from 98.6% to 106.0%. Conclusion The method has high sensitivity, good repeatability, accurate and reliable results, high degree of automation and easy operation. It is suitable for the batch determination of beryllium concentration in the practitioners.