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已有研究表明,细胞损伤后的修复与原癌基因的适当调节有关[Kidney Int,1991.39:401]。为了解急性缺血性肾损伤分子调控水平的变化,我们应用Northern杂交技术,在大鼠缺血再灌注肾组织中研究了几种原癌基因的表达,以探讨原癌基因在缺血肾脏损伤修复中的可能作用。 一、材料与方法 选用纯种SD雄性大鼠5只,4月龄,体重250～300g。实验组12只,腹腔麻醉下行右肾切除,左肾动脉夹闭45分钟,分别于再灌注1、4、8、24小时摘取左肾剥除包膜及肾上腺,液氮冷冻待用。对
It has been reported that repair after cell injury is related to the proper regulation of proto-oncogenes [Kidney Int, 1991.39: 401]. To understand the molecular changes in the regulation of acute ischemic renal damage, we used Northern hybridization to study the expression of several oncogenes in the rat model of ischemia-reperfusion injury in order to explore the role of protooncogene in ischemic renal damage Possible role in repair. First, materials and methods Selection of pure SD male rats 5, 4 months old, weighing 250 ~ 300g. Twelve rats in the experimental group underwent right nephrectomy under celiac anesthesia. The left renal artery was closed for 45 minutes. The left renal extermination capsule and adrenal glands were removed at 1, 4, 8 and 24 hours after reperfusion. Correct