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目的初步探讨亚急性高原低氧对移居大鼠海马神经元凋亡和CA1区半胱天冬蛋白酶-3(Caspase-3)表达的影响及其机制。方法将48只清洁级健康成年Wistar雄性大鼠随机分为2组,低海拔对照组(海拔400m),亚急性高原组(海拔4300m);在不同海拔喂养30d后,取海马组织,利用流式细胞仪检测海马神经元凋亡率,通过免疫组织化学法观察CA1区神经元Caspase-3的表达变化,并测定海马组织MDA含量、SOD活力、Ca2+-ATP酶活力和钙含量。结果与低海拔对照组相比,亚急性高原组大鼠海马神经元凋亡率、CA1区神经元Caspase-3免疫组化阳性细胞数、MDA含量、钙含量均较高,而Ca2+-ATP酶活力降低,差异均有统计学意义(P<0.01)。两组SOD活力间差异无统计学意义。结论亚急性高原低氧可诱导移居大鼠海马神经元凋亡,其作用机制可能与其降低Ca2+-ATP酶活力、增加组织钙含量,使自由基含量升高有关。
Objective To investigate the effects of subacute plateau hypoxia on the apoptosis of hippocampus and the expression of Caspase-3 in hippocampus CA1 subregion in rats. Methods Forty-eight healthy adult Wistar male rats were randomly divided into two groups: low altitude control group (altitude 400m), subacute plateau group (altitude 4300m); at different altitudes for 30 days, The apoptosis rate of hippocampal neurons was detected by cytometry. The expression of Caspase-3 in CA1 neurons was observed by immunohistochemical method. The content of MDA, the activity of SOD, the activity of Ca2 + -ATPase and the content of calcium in hippocampus were determined. Results Compared with the control group at low altitude, the apoptotic rate of hippocampal neurons, the number of Caspase-3 positive cells, the content of MDA and the content of Ca2 + Vitality decreased, the differences were statistically significant (P <0.01). There was no significant difference in SOD activity between the two groups. Conclusion Subacute plateau hypoxia can induce the apoptosis of hippocampal neurons in migrating rats. The mechanism may be related to the decrease of Ca2 + -ATPase activity, increase of calcium content and increase of free radical content.